Pfu polymerase Thermo

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Phusion DNA Polymerases | Thermo Fisher Scientific - SGThe error rate, determined by a modified lacI-based method [1], is approximately 50-fold lower than that of Taq DNA polymerase and 6-fold lower than that of Pfu ...[PDF] Pfu DNA Polymerase, recombinant - Thermo Fisher Scientific2016年6月3日 · Thermo Scientific™ Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus.Phusion™ High-Fidelity DNA Polymerase (2 U/µL) - Thermo Fisher ...Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq,  ...Taq DNA Polymerase, recombinant (5 U/µL) - Thermo Fisher ScientificThermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes ...DNA Polymerase—Four Key Characteristics for PCR | Thermo ...Since the use of Taq DNA polymerase in early PCR protocols, significant improvements have been made specifically in the specificity, thermostability, fidelity, ...Modified DNA polymerases for PCR troubleshooting - NCBI - NIH2016年10月28日 · The Taq DNA polymerase is an example of an enzyme in which ... and thermal stability, while the replication fidelity is similar to Pfu DNA polymerase (2.0 × 10− 6). ... [PubMed] [CrossRef] [Google Scholar]; Gelfand DH, Reichert FL (2001) Mutant chimeric DNA polymerase. ... Facebook · Share on Twitter ...Improving dideoxynucleotide-triphosphate utilisation by the hyper ...Wild-type Pfu–Pol required a ddNTP:dNTP ratio of 30:1; values of 5:1 (Q472H), 1: 3 (L490W), 1:5 ... Thermo-SequenaseTM, a Taq polymerase mutant that lacks exonuclease activity, and contains ... Prober J.M., Trainer,G.L., Dam,R.J., Hobbs, F.W., Robertson,C.W., Zagursky,R.J., Cocuzza,A.J., ... Facebook; Share on Twitter  ...PrimeSTAR GXL DNA Polymerase - Takara BioPrimeSTAR GXL DNA Polymerase is a high-fidelity PCR enzyme for superior ... I tried different Taq to amplify a fragment with a high GC content (70%) and this is ...[PDF] High-fidelity amplification using a thermostable DNA polymerase ...These studies demonstrate that the error rate per nucleotide induced in the I82 known detectable sites of the lucl gene was. 1.6 x lo-' for Pfu DNA polymerase, a  ...Rapid PCR site-directed mutagenesis. - Genome ResearchWeiner, M.P., Costa, G.L. Schoettlin, W. Cline, J. Mathur, E. and Bauer. ... Polishing with T4 or Pfu polymerase increases the efficiency of cloning PCR fragments. ... Reddit; Add to Twitter ... reveals a molecular mechanism to regulate RNA editing during development · Thermal stress accelerates Arabidopsis thaliana mutation ...


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